Install PyGTK via Homebrew and virtualenv

install-pygtk.sh

# This LOOKS pretty straightforward, but it took awhile to sort out issues with
# py2cairo and pygobject, so I hope I've saved you some time :-)
#
# This assumes you already subscribe to a nice clean virtualenvwrapper workflow
# -- see https://gist.github.com/771394 if you need advice on getting there.
# There are some optional dependencies omitted, so if you're going to be doing
# heavy development with these libs, you may want to look into them.
#
# We go to some configure option pains to avoid polluting the system-level
# Python, and `brew link`ing Cairo which is keg-only by default.

brew install cairo && brew install gtk+
# Have a coffee. Better yet, a meal.

mkvirtualenv --no-site-packages pygtk
workon pygtk

# NOTE: pycairo uses a nonstandard build system, the autotools approach is
# officially unsupported. I couldn't get the latest version (1.10.0 at time of
# writing) to work with either approach, so I punted and used autotools here
# with the next-most-recent version.
curl -O http://cairographics.org/releases/py2cairo-1.8.10.tar.gz
tar xzf py2cairo-1.8.10.tar.gz && cd py2cairo-1.8.10
./configure --prefix=$VIRTUAL_ENV PKG_CONFIG_PATH=/usr/local/lib/pkgconfig:/usr/local/Cellar/cairo/1.10.2/lib/pkgconfig
make
make install

# pygobject
# introspection no worky: https://github.com/mxcl/homebrew/pull/4353
curl -O http://ftp.gnome.org/pub/GNOME/sources/pygobject/2.28/pygobject-2.28.6.tar.bz2
tar xf pygobject-2.28.6.tar.bz2 && cd pygobject-2.28.6
./configure --prefix=$VIRTUAL_ENV --disable-introspection
make
make install

# pygtk
curl -O http://ftp.gnome.org/pub/GNOME/sources/pygtk/2.24/pygtk-2.24.0.tar.bz2
tar xf pygtk-2.24.0.tar.bz2 && cd pygtk-2.24.0
./configure --prefix=$VIRTUAL_ENV PKG_CONFIG_PATH=/usr/local/lib/pkgconfig:$VIRTUAL_ENV/lib/pkgconfig
make    # moar coffee nao
make install

# There's a short test script you can run from the REPL here, if you want to
# verify that things are working:
#
#   http://faq.pygtk.org/index.py?file=faq21.001.htp&req=show
#

openxenmanager.sh

#!/bin/sh
# ~/bin/openxenmanager

source ~/.virtualenvs/pygtk/bin/activate
cd $HOME/src/python/openxenmanager
python window.py
deactivate

After doing
brew install pygtk and export PYTHONPATH=/usr/local/lib/python2.7/site-packages

I get

Traceback (most recent call last):
  File "window.py", line 45, in <module>
    import gtk
ImportError: No module named gtk

:(

I have the same issue :(. How can we take care of this?

File "window.py" asks for python 2.5 and gtk 2.0

open window.py and change the request line of python 2.5 to the version you have...
i have python 2.7 and it worked after that (i hade to change the permission of file and folder to do so ^_^)

brew no longer seems to have a formula for pygtx

[joule] ~ [1.9.3-p194] brew update
Updated Homebrew from 93fe0378 to ce39c3f3.
==> Updated Formulae
adobe-air-sdk  doxygen        zeromq
[joule] ~ [1.9.3-p194] brew info pygtx
Error: No available formula for pygtx 
[joule] ~ [1.9.3-p194] 

@jedschneider - surely you want pygtk not pygtx

This may help some people:
Copy these files to /usr/local/Homebrew/Library/Taps/homebrew/homebrew-core/Formula

pygobject.rb
---Begin---

class Pygobject < Formula
  desc "GLib/GObject/GIO Python bindings for Python 2"
  homepage "https://wiki.gnome.org/Projects/PyGObject"
  url "https://download.gnome.org/sources/pygobject/2.28/pygobject-2.28.7.tar.xz"
  sha256 "bb9d25a3442ca7511385a7c01b057492095c263784ef31231ffe589d83a96a5a"
  revision 2

  bottle do
    cellar :any
    sha256 "2dbdab3cd94b10eae121c1e8460085b84c1908b487150e486005dd125f99890d" => :catalina
    sha256 "caf4b79e8454b58bb67e73a6f06853d7a410c1fa9b31478c6ac389424ca690bb" => :mojave
    sha256 "04550d558f335fd6431ee3c124ba19011ec4284e7584eff343ad6b78483472a5" => :high_sierra
    sha256 "fbe187ff2aa28f4e9f57e1e3f8f69df8e69da6406833b3dd9a976f5bae267ee7" => :sierra
  end

  depends_on "pkg-config" => :build
  depends_on "glib"
  depends_on "python@2"

  patch do
    url "https://raw.githubusercontent.com/Homebrew/formula-patches/85fa66a9/pygobject/2.28.7.diff"
    sha256 "ada3da43c84410cc165d8547ad3c7809435e09c9e8539882860d97cd1ce922b2"
  end

  def install
    system "./configure", "--disable-dependency-tracking",
                          "--prefix=#{prefix}",
                          "--disable-introspection"
    system "make", "install"
    (lib/"python2.7/site-packages/pygtk.pth").append_lines <<~EOS
      #{HOMEBREW_PREFIX}/lib/python2.7/site-packages/gtk-2.0
    EOS
  end

  test do
    system Formula["python@2"].opt_bin/"python2.7", "-c", "import dsextras"
  end
end

---End---

pygtk.rb
---Begin---

class Pygtk < Formula
  desc "GTK+ bindings for Python"
  homepage "http://www.pygtk.org/"
  url "https://download.gnome.org/sources/pygtk/2.24/pygtk-2.24.0.tar.bz2"
  sha256 "cd1c1ea265bd63ff669e92a2d3c2a88eb26bcd9e5363e0f82c896e649f206912"
  revision 3

  bottle do
    cellar :any
    rebuild 2
    sha256 "12bab3d76587659b38e56867c9b359941803275716896e2936cd3e8029cf5f3f" => :catalina
    sha256 "87f89d246e3a779381ec2efdee7ee2b69fda464f38a59dd8e14304435d759419" => :mojave
    sha256 "969cef803e110b2767c6d3ade304b92d7f23a02ff7eb4030772b69b52df7c3b2" => :high_sierra
  end

  depends_on "pkg-config" => :build
  depends_on "atk"
  depends_on "glib"
  depends_on "gtk+"
  depends_on "libglade"
  depends_on "py2cairo"
  depends_on "pygobject"

  # Allow building with recent Pango, where some symbols were removed
  patch do
    url "https://raw.githubusercontent.com/Homebrew/formula-patches/85fa66a9/pygtk/2.24.0.diff"
    sha256 "ec480cff20082c41d9015bb7f7fc523c27a2c12a60772b2c55222e4ba0263dde"
  end

  def install
    ENV.append "CFLAGS", "-ObjC"
    system "./configure", "--disable-dependency-tracking",
                          "--prefix=#{prefix}"
    system "make", "install"

    # Fixing the pkgconfig file to find codegen, because it was moved from
    # pygtk to pygobject. But our pkgfiles point into the cellar and in the
    # pygtk-cellar there is no pygobject.
    inreplace lib/"pkgconfig/pygtk-2.0.pc", "codegendir=${datadir}/pygobject/2.0/codegen", "codegendir=#{HOMEBREW_PREFIX}/share/pygobject/2.0/codegen"
    inreplace bin/"pygtk-codegen-2.0", "exec_prefix=${prefix}", "exec_prefix=#{Formula["pygobject"].opt_prefix}"
  end

  test do
    (testpath/"codegen.def").write("(define-enum asdf)")
    system "#{bin}/pygtk-codegen-2.0", "codegen.def"
  end
end

---End---